A versatile toolkit for CRISPR-Cas13-based RNA manipulation in Drosophila

A versatile toolkit for CRISPR-Cas13-based RNA manipulation in Drosophila

Abstract Advances in CRISPR technology have immensely improved our ability to manipulate nucleic acids, and the recent discovery of the RNA-targeting endonuclease Cas13 adds even further functionality. Here, we show that Cas13 works efficiently in Drosophila, both ex vivo and in vivo. We test 44 dif...

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Bibliographic Details
Main Authors: Nhan Huynh, Noah Depner, Raegan Larson, Kirst King-Jones
Format: Article
Language:English
Published: BMC 2020-11-01
Series:Genome Biology
Subjects:
CRISPR
Cas13
CasRX
Drosophila
RNA manipulation
crRNA design
Online Access:http://link.springer.com/article/10.1186/s13059-020-02193-y

Internet

http://link.springer.com/article/10.1186/s13059-020-02193-y

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