An Optimized Fluorescence-Based Bidimensional Immunoproteomic Approach for Accurate Screening of Autoantibodies.

An Optimized Fluorescence-Based Bidimensional Immunoproteomic Approach for Accurate Screening of Autoantibodies.

Serological proteome analysis (SERPA) combines classical proteomic technology with effective separation of cellular protein extracts on two-dimensional gel electrophoresis, western blotting, and identification of the antigenic spot of interest by mass spectrometry. A critical point is related to the...

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Bibliographic Details
Main Authors: Virginie Dutoit-Lefèvre, Sylvain Dubucquoi, David Launay, Vincent Sobanski, Patricia Dussart, Philippe Chafey, Cédric Broussard, Sophie Duban-Deweer, Patrick Vermersch, Lionel Prin, Didier Lefranc
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2015-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4489013?pdf=render

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http://europepmc.org/articles/PMC4489013?pdf=render

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