Validation and standardization of DNA extraction and library construction methods for metagenomics-based human fecal microbiome measurements

Validation and standardization of DNA extraction and library construction methods for metagenomics-based human fecal microbiome measurements

Abstract Background Validation and standardization of methodologies for microbial community measurements by high-throughput sequencing are needed to support human microbiome research and its industrialization. This study set out to establish standards-based solutions to improve the accuracy and repr...

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Bibliographic Details
Main Authors: Dieter M. Tourlousse, Koji Narita, Takamasa Miura, Mitsuo Sakamoto, Akiko Ohashi, Keita Shiina, Masami Matsuda, Daisuke Miura, Mamiko Shimamura, Yoshifumi Ohyama, Atsushi Yamazoe, Yoshihito Uchino, Keishi Kameyama, Shingo Arioka, Jiro Kataoka, Takayoshi Hisada, Kazuyuki Fujii, Shunsuke Takahashi, Miho Kuroiwa, Masatomo Rokushima, Mitsue Nishiyama, Yoshiki Tanaka, Takuya Fuchikami, Hitomi Aoki, Satoshi Kira, Ryo Koyanagi, Takeshi Naito, Morie Nishiwaki, Hirotaka Kumagai, Mikiko Konda, Ken Kasahara, Moriya Ohkuma, Hiroko Kawasaki, Yuji Sekiguchi, Jun Terauchi
Format: Article
Language:English
Published: BMC 2021-04-01
Series:Microbiome
Subjects:
Human microbiome
Metagenomics
Gut microbiota
Standardization
Accuracy, reproducibility, and comparability
Industrialization
Online Access:https://doi.org/10.1186/s40168-021-01048-3

Internet

https://doi.org/10.1186/s40168-021-01048-3

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