DEVELOPMENT OF NEW SEQUENCING TECHNOLOGIES AND THEIR APPLICATION IN GENOME ANALYSIS OF DOMESTIC ANIMALS

• Main Authors: Kristina Gvozdanović, Vice Čuljak, Polonca Margeta
• Format: Article
• Language: English
• Published: Faculty of Agrobitechnical Sciences Osijek 2015-12-01
• Series: Poljoprivreda
• Subjects: sequencing; Sanger sequencing method; next generation sequencing; domestic animal genome analysis
• Online Access: http://poljoprivreda.pfos.hr/upload/publications/poljoprivreda-21-2-11.pdf

Sequencing and detailed study of the genom of domestic animals began in the middle of the last century. It was primarily referred to development of the first generation sequencing methods, i.e. Sanger sequencing method. Next generation sequencing methods are currently the most common methods in the analysis of domestic animals genom. The application of these methods gave us up to 100 time more data in comparison with Sanger method. Analyses including RNA sequencing, genotyping of whole genome, immunoprecipitation associated with DNA microarrays, detection ofmutations and inherited diseases, sequencing ofthemitochondrial genome and many others have been conducted with development and application of new sequencing methods since 2005 until today. Application of new sequencing methods in the analysis ofdomestic animal genome provides better understanding of the genetic basis for important production traits which could help in improving the livestock production.