Development and evaluation of a milk protein transcript depletion method for differential transcriptome analysis in mammary gland tissue

Development and evaluation of a milk protein transcript depletion method for differential transcriptome analysis in mammary gland tissue

Abstract Background In the mammary gland transcriptome of lactating dairy cows genes encoding milk proteins are highly abundant, which can impair the detection of lowly expressed transcripts and can bias the outcome in global transcriptome analyses. Therefore, the aim of this study was to develop an...

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Bibliographic Details
Main Authors: Johanna Brodhagen, Rosemarie Weikard, Ulrike Thom, Annika Heimes, Juliane Günther, Frieder Hadlich, Holm Zerbe, Wolfgang Petzl, Marie M. Meyerholz, Martina Hoedemaker, Hans-Joachim Schuberth, Susanne Engelmann, Christa Kühn
Format: Article
Language:English
Published: BMC 2019-05-01
Series:BMC Genomics
Subjects:
RNA depletion
RNA-sequencing
Mammary gland
Lactation
Mastitis
Online Access:http://link.springer.com/article/10.1186/s12864-019-5781-3

Internet

http://link.springer.com/article/10.1186/s12864-019-5781-3

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