Synthetic Standards Combined With Error and Bias Correction Improve the Accuracy and Quantitative Resolution of Antibody Repertoire Sequencing in Human Naïve and Memory B Cells

Synthetic Standards Combined With Error and Bias Correction Improve the Accuracy and Quantitative Resolution of Antibody Repertoire Sequencing in Human Naïve and Memory B Cells

High-throughput sequencing of immunoglobulin (Ig) repertoires (Ig-seq) is a powerful method for quantitatively interrogating B cell receptor sequence diversity. When applied to human repertoires, Ig-seq provides insight into fundamental immunological questions, and can be implemented in diagnostic a...

Full description

Bibliographic Details
Main Authors: Simon Friedensohn, John M. Lindner, Vanessa Cornacchione, Mariavittoria Iazeolla, Enkelejda Miho, Andreas Zingg, Simon Meng, Elisabetta Traggiai, Sai T. Reddy
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-06-01
Series:Frontiers in Immunology
Subjects:
antibody repertoire
next-generation sequencing
unique molecular identifiers
B cells
bioinformatics
systems immunology
Online Access:https://www.frontiersin.org/article/10.3389/fimmu.2018.01401/full

Internet

https://www.frontiersin.org/article/10.3389/fimmu.2018.01401/full

Similar Items