A low-cost library construction protocol and data analysis pipeline for Illumina-based strand-specific multiplex RNA-seq.

A low-cost library construction protocol and data analysis pipeline for Illumina-based strand-specific multiplex RNA-seq.

The emergence of NextGen sequencing technology has generated much interest in the exploration of transcriptomes. Currently, Illumina Inc. (San Diego, CA) provides one of the most widely utilized sequencing platforms for gene expression analysis. While Illumina reagents and protocols perform adequate...

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Bibliographic Details
Main Authors: Lin Wang, Yaqing Si, Lauren K Dedow, Ying Shao, Peng Liu, Thomas P Brutnell
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2011-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3198403?pdf=render

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http://europepmc.org/articles/PMC3198403?pdf=render

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