Marine Fish Primary Hepatocyte Isolation and Culture: New Insights to Enzymatic Dissociation Pancreatin Digestion

Primary cell cultures from wild organisms have been gaining relevance in ecotoxicology as they are considered more sensitive than immortalized cell lines and retain the biochemical pathways found in vivo. In this study, the efficacy of two methods for primary hepatocyte cell isolation was compared u...

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Bibliographic Details
Main Authors: Neusa Figueiredo, Beatriz Matos, Mário Diniz, Vasco Branco, Marta Martins
Format: Article
Language:English
Published: MDPI AG 2021-02-01
Series:International Journal of Environmental Research and Public Health
Subjects:
Online Access:https://www.mdpi.com/1660-4601/18/4/1380