Molecular beacon-based real-time PCR detection of primary isolates of <it>Salmonella </it>Typhimurium and <it>Salmonella </it>Enteritidis in environmental and clinical samples

<p>Abstract</p> <p>Background</p> <p>A fast and simple two-step multiplex real-time PCR assay has been developed to replace the traditional, laborious Salmonella serotyping procedure. Molecular beacons were incorporated into the assay as probes for target DNA. Target se...

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Bibliographic Details
Main Authors: Emmanuel Maria A, Demetriou Victoria L, Hadjinicolaou Andreas V, Kakoyiannis Charalambos K, Kostrikis Leondios G
Format: Article
Language:English
Published: BMC 2009-05-01
Series:BMC Microbiology
Online Access:http://www.biomedcentral.com/1471-2180/9/97