Site-specific labeling of neurotrophins and their receptors via short and versatile peptide tags.

We present a toolbox for the study of molecular interactions occurring between NGF and its receptors. By means of a suitable insertional mutagenesis method we show the insertion of an 8 amino acid tag (A4) into the sequence of NGF and of 12 amino acid tags (A1 and S6) into the sequence of TrkA and P...

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Bibliographic Details
Main Authors: Laura Marchetti, Teresa De Nadai, Fulvio Bonsignore, Mariantonietta Calvello, Giovanni Signore, Alessandro Viegi, Fabio Beltram, Stefano Luin, Antonino Cattaneo
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4245215?pdf=render