Arterial endothelium creates a permissive niche for expansion of human cord blood hematopoietic stem and progenitor cells
Abstract Background Although cord blood (CB) offers promise for treatment of patients with high-risk hematological malignancies and immune disorders, the limited numbers of hematopoietic stem cell (HSC)/progenitor cell in a CB unit and straitened circumstances in expanding ex vivo make it quite chal...
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doaj-dc9e7a42d8754a7d800ed3c55e0243dc2020-11-25T03:48:12ZengBMCStem Cell Research & Therapy1757-65122020-08-0111111310.1186/s13287-020-01880-8Arterial endothelium creates a permissive niche for expansion of human cord blood hematopoietic stem and progenitor cellsHuilin Li0Haiyun Pei1Sihan Wang2Bowen Zhang3Zeng Fan4Yiming Liu5Xiaoyan Xie6Zhou Yang7Lei Xu8Yali Jia9Yun Bai10Yi Han11Lin Chen12Lijuan He13Xue Nan14Wen Yue15Xuetao Pei16Stem Cell and Regenerative Medicine Lab, Institute of Health Service and Transfusion MedicineExperimental Hematology and Biochemistry Lab, Beijing Institute of Radiation MedicineStem Cell and Regenerative Medicine Lab, Institute of Health Service and Transfusion MedicineExperimental Hematology and Biochemistry Lab, Beijing Institute of Radiation MedicineStem Cell and Regenerative Medicine Lab, Institute of Health Service and Transfusion MedicineStem Cell and Regenerative Medicine Lab, Institute of Health Service and Transfusion MedicineStem Cell and Regenerative Medicine Lab, Institute of Health Service and Transfusion MedicineStem Cell and Regenerative Medicine Lab, Institute of Health Service and Transfusion MedicineStem Cell and Regenerative Medicine Lab, Institute of Health Service and Transfusion MedicineExperimental Hematology and Biochemistry Lab, Beijing Institute of Radiation MedicineStem Cell and Regenerative Medicine Lab, Institute of Health Service and Transfusion MedicineSouth China Research Center for Stem Cell & Regenerative Medicine, SCIBStem Cell and Regenerative Medicine Lab, Institute of Health Service and Transfusion MedicineStem Cell and Regenerative Medicine Lab, Institute of Health Service and Transfusion MedicineStem Cell and Regenerative Medicine Lab, Institute of Health Service and Transfusion MedicineStem Cell and Regenerative Medicine Lab, Institute of Health Service and Transfusion MedicineStem Cell and Regenerative Medicine Lab, Institute of Health Service and Transfusion MedicineAbstract Background Although cord blood (CB) offers promise for treatment of patients with high-risk hematological malignancies and immune disorders, the limited numbers of hematopoietic stem cell (HSC)/progenitor cell in a CB unit and straitened circumstances in expanding ex vivo make it quite challenging to develop the successful cell therapies. Methods In this study, a novel strategy has been developed to support ex vivo expansion of hematopoietic stem and progenitor cells (HSPCs) by coculture with engineered human umbilical arterial endothelial cells (HuAECs-E4orf1-GFP), which expresses E4ORF1 stably by using a retroviral system. Results Coculture of CD34+ hCB cells with HuAECs-E4orf1-GFP resulted in generation of considerably more total nucleated cells, CD34+CD38−, and CD34+CD38−CD90+ HSPCs in comparison with that of cytokines alone or that of coculture with human umbilical vein endothelial cells (HuVECs) after 14-day amplification. The in vitro multilineage differentiation potential and in vivo repopulating capacity of the expanded hematopoietic cells cocultured with HuAECs-E4orf1-GFP were also markedly enhanced compared with the other two control groups. DLL4, a major determinant of arterial endothelial cell (EC) identity, was associated with CD34+ hCB cells amplified on HuAECs-E4orf1-GFP. Conclusions Collectively, we demonstrated that HuAECs acted as a permissive niche in facilitating expansion of HSPCs. Our study further implicated that the crucial factors and related pathways presented in HuAECs may give a hint to maintain self-renewal of bona fide HSCs.http://link.springer.com/article/10.1186/s13287-020-01880-8Arterial endothelial cellsHematopoietic stem and progenitor cellsExpansionNicheTransplantation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Huilin Li Haiyun Pei Sihan Wang Bowen Zhang Zeng Fan Yiming Liu Xiaoyan Xie Zhou Yang Lei Xu Yali Jia Yun Bai Yi Han Lin Chen Lijuan He Xue Nan Wen Yue Xuetao Pei |
spellingShingle |
Huilin Li Haiyun Pei Sihan Wang Bowen Zhang Zeng Fan Yiming Liu Xiaoyan Xie Zhou Yang Lei Xu Yali Jia Yun Bai Yi Han Lin Chen Lijuan He Xue Nan Wen Yue Xuetao Pei Arterial endothelium creates a permissive niche for expansion of human cord blood hematopoietic stem and progenitor cells Stem Cell Research & Therapy Arterial endothelial cells Hematopoietic stem and progenitor cells Expansion Niche Transplantation |
author_facet |
Huilin Li Haiyun Pei Sihan Wang Bowen Zhang Zeng Fan Yiming Liu Xiaoyan Xie Zhou Yang Lei Xu Yali Jia Yun Bai Yi Han Lin Chen Lijuan He Xue Nan Wen Yue Xuetao Pei |
author_sort |
Huilin Li |
title |
Arterial endothelium creates a permissive niche for expansion of human cord blood hematopoietic stem and progenitor cells |
title_short |
Arterial endothelium creates a permissive niche for expansion of human cord blood hematopoietic stem and progenitor cells |
title_full |
Arterial endothelium creates a permissive niche for expansion of human cord blood hematopoietic stem and progenitor cells |
title_fullStr |
Arterial endothelium creates a permissive niche for expansion of human cord blood hematopoietic stem and progenitor cells |
title_full_unstemmed |
Arterial endothelium creates a permissive niche for expansion of human cord blood hematopoietic stem and progenitor cells |
title_sort |
arterial endothelium creates a permissive niche for expansion of human cord blood hematopoietic stem and progenitor cells |
publisher |
BMC |
series |
Stem Cell Research & Therapy |
issn |
1757-6512 |
publishDate |
2020-08-01 |
description |
Abstract Background Although cord blood (CB) offers promise for treatment of patients with high-risk hematological malignancies and immune disorders, the limited numbers of hematopoietic stem cell (HSC)/progenitor cell in a CB unit and straitened circumstances in expanding ex vivo make it quite challenging to develop the successful cell therapies. Methods In this study, a novel strategy has been developed to support ex vivo expansion of hematopoietic stem and progenitor cells (HSPCs) by coculture with engineered human umbilical arterial endothelial cells (HuAECs-E4orf1-GFP), which expresses E4ORF1 stably by using a retroviral system. Results Coculture of CD34+ hCB cells with HuAECs-E4orf1-GFP resulted in generation of considerably more total nucleated cells, CD34+CD38−, and CD34+CD38−CD90+ HSPCs in comparison with that of cytokines alone or that of coculture with human umbilical vein endothelial cells (HuVECs) after 14-day amplification. The in vitro multilineage differentiation potential and in vivo repopulating capacity of the expanded hematopoietic cells cocultured with HuAECs-E4orf1-GFP were also markedly enhanced compared with the other two control groups. DLL4, a major determinant of arterial endothelial cell (EC) identity, was associated with CD34+ hCB cells amplified on HuAECs-E4orf1-GFP. Conclusions Collectively, we demonstrated that HuAECs acted as a permissive niche in facilitating expansion of HSPCs. Our study further implicated that the crucial factors and related pathways presented in HuAECs may give a hint to maintain self-renewal of bona fide HSCs. |
topic |
Arterial endothelial cells Hematopoietic stem and progenitor cells Expansion Niche Transplantation |
url |
http://link.springer.com/article/10.1186/s13287-020-01880-8 |
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