Efficient Generation and Correction of Mutations in Human iPS Cells Utilizing mRNAs of CRISPR Base Editors and Prime Editors

Efficient Generation and Correction of Mutations in Human iPS Cells Utilizing mRNAs of CRISPR Base Editors and Prime Editors

In contrast to CRISPR/Cas9 nucleases, CRISPR base editors (BE) and prime editors (PE) enable predefined nucleotide exchanges in genomic sequences without generating DNA double strand breaks. Here, we employed BE and PE mRNAs in conjunction with chemically synthesized sgRNAs and pegRNAs for efficient...

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Bibliographic Details
Main Authors: Duran Sürün, Aksana Schneider, Jovan Mircetic, Katrin Neumann, Felix Lansing, Maciej Paszkowski-Rogacz, Vanessa Hänchen, Min Ae Lee-Kirsch, Frank Buchholz
Format: Article
Language:English
Published: MDPI AG 2020-05-01
Series:Genes
Subjects:
CRISPR/Cas9
base editors
prime editors
human induced pluripotent stem cells
mRNA
Online Access:https://www.mdpi.com/2073-4425/11/5/511

Internet

https://www.mdpi.com/2073-4425/11/5/511

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