Multi-focus microscope with HiLo algorithm for fast 3-D fluorescent imaging.

In this paper, we present a new multi-focus microscope (MFM) system based on a phase mask and HiLo algorithm, achieving high-speed (20 volumes per second), high-resolution, low-noise 3-D fluorescent imaging. During imaging, the emissions from the specimen at nine different depths are simultaneously...

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Bibliographic Details
Main Authors: Wei Lin, Dongping Wang, Yunlong Meng, Shih-Chi Chen
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2019-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0222729