Comparison of two commercial broad-range PCR and sequencing assays for identification of bacteria in culture-negative clinical samples

Comparison of two commercial broad-range PCR and sequencing assays for identification of bacteria in culture-negative clinical samples

Abstract Background Culturing has long been the gold standard for detecting aetiologic agents in bacterial infections. In some cases, however, culturing fails to detect the infection. To further investigate culture-negative samples, amplification and subsequent sequencing of the 16S rRNA gene is oft...

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Bibliographic Details
Main Authors: Camilla Stavnsbjerg, Niels Frimodt-Møller, Claus Moser, Thomas Bjarnsholt
Format: Article
Language:English
Published: BMC 2017-03-01
Series:BMC Infectious Diseases
Subjects:
Culture-negative samples
Molecular diagnostics
Universal Microbe Detection
16S PCR
Online Access:http://link.springer.com/article/10.1186/s12879-017-2333-9

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http://link.springer.com/article/10.1186/s12879-017-2333-9

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