Cloning of intronic sequence within DsRed2 increased the number of cells expressing red fluorescent protein

Cloning of intronic sequence within DsRed2 increased the number of cells expressing red fluorescent protein

Aim: Cloning of artificial intronic sequence within the open reading frame (ORF) of DsRed2 gene. Method: Splice prediction software was used to analyze DsRed2 sequence to find an ideal site for cloning artificial intronic sequence. Intron was cloned within DsRed2 using cyclic ligation assembly. Flow...

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Bibliographic Details
Main Authors: Rishikaysh V. Pisal, Hana Hrebikova, Jana Chvatalova, Tomas Soukup, Stanislav Filip, Jaroslav Mokry
Format: Article
Language:English
Published: Palacký University Olomouc, Faculty of Medicine and Dentistry 2017-12-01
Series:Biomedical Papers
Subjects:
dsred2
cyclic ligation assembly (cla)
intron cloned dsred2
Online Access:https://biomed.papers.upol.cz/artkey/bio-201704-0004_Cloning_of_intronic_sequence_within_DsRed2_increased_the_number_of_cells_expressing_red_fluorescent_protein.php

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https://biomed.papers.upol.cz/artkey/bio-201704-0004_Cloning_of_intronic_sequence_within_DsRed2_increased_the_number_of_cells_expressing_red_fluorescent_protein.php

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