Investigating Immunization With Nucleotide Enzymes of Schistosoma mansoni: Nucleoside Diphosphate Kinase and Adenylosuccinate Lyase as New Antigenic Targets Against Schistosomiasis
Schistosomiasis, caused by Schistosoma mansoni trematode worm, affects more than 1.5 million people in Brazil. The current treatment consists in the administration of Praziquantel, the only medicine used for treatment for more than 40 years. Some of the limitations of this drug consist in its inacti...
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Frontiers Media S.A.
2020-09-01
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Series: | Frontiers in Immunology |
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Online Access: | https://www.frontiersin.org/article/10.3389/fimmu.2020.569988/full |
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Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Túlio di Orlando Cagnazzo Camila Tita Nogueira Cynthia Aparecida de Castro Débora Meira Neris Ana Carolina Maragno Fattori Ricardo de Oliveira Correia Yulli Roxenne Albuquerque Bruna Dias de Lima Fragelli Tiago Manuel Fernandes Mendes Silmara Marques Allegretti Edson Garcia Soares Larissa Romanello Juliana Roberta Torini Humberto D’Muniz Pereira Fernanda de Freitas Anibal |
spellingShingle |
Túlio di Orlando Cagnazzo Camila Tita Nogueira Cynthia Aparecida de Castro Débora Meira Neris Ana Carolina Maragno Fattori Ricardo de Oliveira Correia Yulli Roxenne Albuquerque Bruna Dias de Lima Fragelli Tiago Manuel Fernandes Mendes Silmara Marques Allegretti Edson Garcia Soares Larissa Romanello Juliana Roberta Torini Humberto D’Muniz Pereira Fernanda de Freitas Anibal Investigating Immunization With Nucleotide Enzymes of Schistosoma mansoni: Nucleoside Diphosphate Kinase and Adenylosuccinate Lyase as New Antigenic Targets Against Schistosomiasis Frontiers in Immunology schistosomiasis Schistosoma mansoni Nucleoside Diphosphate Kinase Adenylosuccinate Lyase immunization |
author_facet |
Túlio di Orlando Cagnazzo Camila Tita Nogueira Cynthia Aparecida de Castro Débora Meira Neris Ana Carolina Maragno Fattori Ricardo de Oliveira Correia Yulli Roxenne Albuquerque Bruna Dias de Lima Fragelli Tiago Manuel Fernandes Mendes Silmara Marques Allegretti Edson Garcia Soares Larissa Romanello Juliana Roberta Torini Humberto D’Muniz Pereira Fernanda de Freitas Anibal |
author_sort |
Túlio di Orlando Cagnazzo |
title |
Investigating Immunization With Nucleotide Enzymes of Schistosoma mansoni: Nucleoside Diphosphate Kinase and Adenylosuccinate Lyase as New Antigenic Targets Against Schistosomiasis |
title_short |
Investigating Immunization With Nucleotide Enzymes of Schistosoma mansoni: Nucleoside Diphosphate Kinase and Adenylosuccinate Lyase as New Antigenic Targets Against Schistosomiasis |
title_full |
Investigating Immunization With Nucleotide Enzymes of Schistosoma mansoni: Nucleoside Diphosphate Kinase and Adenylosuccinate Lyase as New Antigenic Targets Against Schistosomiasis |
title_fullStr |
Investigating Immunization With Nucleotide Enzymes of Schistosoma mansoni: Nucleoside Diphosphate Kinase and Adenylosuccinate Lyase as New Antigenic Targets Against Schistosomiasis |
title_full_unstemmed |
Investigating Immunization With Nucleotide Enzymes of Schistosoma mansoni: Nucleoside Diphosphate Kinase and Adenylosuccinate Lyase as New Antigenic Targets Against Schistosomiasis |
title_sort |
investigating immunization with nucleotide enzymes of schistosoma mansoni: nucleoside diphosphate kinase and adenylosuccinate lyase as new antigenic targets against schistosomiasis |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Immunology |
issn |
1664-3224 |
publishDate |
2020-09-01 |
description |
Schistosomiasis, caused by Schistosoma mansoni trematode worm, affects more than 1.5 million people in Brazil. The current treatment consists in the administration of Praziquantel, the only medicine used for treatment for more than 40 years. Some of the limitations of this drug consist in its inactivity against schistosomula and parasite eggs, the appearance of resistant strains and non-prevention against reinfection. Thus, the objective of this study was to evaluate the effect of immunization with recombinant functional enzymes of the purine salvage pathway of S. mansoni, Nucleoside Diphosphate Kinase (NDPK) and Adenylosuccinate Lyase (ADSL), to evaluate the host immune response, as well as the parasite load after vaccination. For this, Balb/c mice were divided into 5 groups: control (uninfected and untreated), non-immunized/infected, NDPK infected, ADSL infected, and NDPK + ADSL infected. Immunized groups received three enzyme dosages, with a 15-day interval between each dose, and after 15 days of the last application the animals were infected with 80 cercariae of S. mansoni. On the 47th day after the infection, fecal eggs were counted and, on the 48th day after the infection, the evaluation of leukocyte response, parasite load, antibody production, cytokines quantification, and histopathological analysis were performed. The results showed that immunizations with NDPK, ADSL or NDPK + ADSL promoted a discreet reduction in eosinophil counts in lavage of peritoneal cavity. All immunized animals showed increased production and secretion of IgG1, IgG2a, and IgE antibodies. Increased production of IL-4 was observed in the group immunized with the combination of both enzymes (NDPK + ADSL). In addition, in all immunized groups there were reductions in egg counts in the liver and intestine, such as reductions in liver granulomas. Thus, we suggest that immunizations with these enzymes could contribute to the reduction of schistosomiasis transmission, besides being important in immunopathogenesis control of the disease. |
topic |
schistosomiasis Schistosoma mansoni Nucleoside Diphosphate Kinase Adenylosuccinate Lyase immunization |
url |
https://www.frontiersin.org/article/10.3389/fimmu.2020.569988/full |
work_keys_str_mv |
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doaj-dd8d3a4337134812a8fa3aae547b367c2020-11-25T03:35:01ZengFrontiers Media S.A.Frontiers in Immunology1664-32242020-09-011110.3389/fimmu.2020.569988569988Investigating Immunization With Nucleotide Enzymes of Schistosoma mansoni: Nucleoside Diphosphate Kinase and Adenylosuccinate Lyase as New Antigenic Targets Against SchistosomiasisTúlio di Orlando Cagnazzo0Camila Tita Nogueira1Cynthia Aparecida de Castro2Débora Meira Neris3Ana Carolina Maragno Fattori4Ricardo de Oliveira Correia5Yulli Roxenne Albuquerque6Bruna Dias de Lima Fragelli7Tiago Manuel Fernandes Mendes8Silmara Marques Allegretti9Edson Garcia Soares10Larissa Romanello11Juliana Roberta Torini12Humberto D’Muniz Pereira13Fernanda de Freitas Anibal14Laboratório de Inflamação e Doenças Infecciosas, Departamento de Morfologia e Patologia, Universidade Federal de São Carlos – UFSCar, São Carlos, BrazilLaboratório de Inflamação e Doenças Infecciosas, Departamento de Morfologia e Patologia, Universidade Federal de São Carlos – UFSCar, São Carlos, BrazilLaboratório de Inflamação e Doenças Infecciosas, Departamento de Morfologia e Patologia, Universidade Federal de São Carlos – UFSCar, São Carlos, BrazilLaboratório de Inflamação e Doenças Infecciosas, Departamento de Morfologia e Patologia, Universidade Federal de São Carlos – UFSCar, São Carlos, BrazilLaboratório de Inflamação e Doenças Infecciosas, Departamento de Morfologia e Patologia, Universidade Federal de São Carlos – UFSCar, São Carlos, BrazilLaboratório de Inflamação e Doenças Infecciosas, Departamento de Morfologia e Patologia, Universidade Federal de São Carlos – UFSCar, São Carlos, BrazilLaboratório de Inflamação e Doenças Infecciosas, Departamento de Morfologia e Patologia, Universidade Federal de São Carlos – UFSCar, São Carlos, BrazilLaboratório de Inflamação e Doenças Infecciosas, Departamento de Morfologia e Patologia, Universidade Federal de São Carlos – UFSCar, São Carlos, BrazilDepartamento de Biologia Animal, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, BrazilDepartamento de Biologia Animal, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, BrazilLaboratório de Citopatologia, Departamento de Patologia e Medicina Legal, Universidade de São Paulo, Ribeirão Preto, BrazilLaboratório de Biologia Estrutural, Instituto de Física de São Carlos, Universidade de São Paulo, São Carlos, BrazilLaboratório de Biologia Estrutural, Instituto de Física de São Carlos, Universidade de São Paulo, São Carlos, BrazilLaboratório de Biologia Estrutural, Instituto de Física de São Carlos, Universidade de São Paulo, São Carlos, BrazilLaboratório de Inflamação e Doenças Infecciosas, Departamento de Morfologia e Patologia, Universidade Federal de São Carlos – UFSCar, São Carlos, BrazilSchistosomiasis, caused by Schistosoma mansoni trematode worm, affects more than 1.5 million people in Brazil. The current treatment consists in the administration of Praziquantel, the only medicine used for treatment for more than 40 years. Some of the limitations of this drug consist in its inactivity against schistosomula and parasite eggs, the appearance of resistant strains and non-prevention against reinfection. Thus, the objective of this study was to evaluate the effect of immunization with recombinant functional enzymes of the purine salvage pathway of S. mansoni, Nucleoside Diphosphate Kinase (NDPK) and Adenylosuccinate Lyase (ADSL), to evaluate the host immune response, as well as the parasite load after vaccination. For this, Balb/c mice were divided into 5 groups: control (uninfected and untreated), non-immunized/infected, NDPK infected, ADSL infected, and NDPK + ADSL infected. Immunized groups received three enzyme dosages, with a 15-day interval between each dose, and after 15 days of the last application the animals were infected with 80 cercariae of S. mansoni. On the 47th day after the infection, fecal eggs were counted and, on the 48th day after the infection, the evaluation of leukocyte response, parasite load, antibody production, cytokines quantification, and histopathological analysis were performed. The results showed that immunizations with NDPK, ADSL or NDPK + ADSL promoted a discreet reduction in eosinophil counts in lavage of peritoneal cavity. All immunized animals showed increased production and secretion of IgG1, IgG2a, and IgE antibodies. Increased production of IL-4 was observed in the group immunized with the combination of both enzymes (NDPK + ADSL). In addition, in all immunized groups there were reductions in egg counts in the liver and intestine, such as reductions in liver granulomas. Thus, we suggest that immunizations with these enzymes could contribute to the reduction of schistosomiasis transmission, besides being important in immunopathogenesis control of the disease.https://www.frontiersin.org/article/10.3389/fimmu.2020.569988/fullschistosomiasisSchistosoma mansoniNucleoside Diphosphate KinaseAdenylosuccinate Lyaseimmunization |