A robust multiplex immunofluorescence and digital pathology workflow for the characterisation of the tumour immune microenvironment

A robust multiplex immunofluorescence and digital pathology workflow for the characterisation of the tumour immune microenvironment

Multiplex immunofluorescence is a powerful tool for the simultaneous detection of tissue‐based biomarkers, revolutionising traditional immunohistochemistry. The Opal methodology allows up to eight biomarkers to be measured concomitantly without cross‐reactivity, permitting identification of differen...

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Bibliographic Details
Main Authors: Amélie Viratham Pulsawatdi, Stephanie G. Craig, Victoria Bingham, Kris McCombe, Matthew P. Humphries, Seedevi Senevirathne, Susan D. Richman, Phil Quirke, Leticia Campo, Enric Domingo, Timothy S. Maughan, Jacqueline A. James, Manuel Salto‐Tellez
Format: Article
Language:English
Published: Wiley 2020-10-01
Series:Molecular Oncology
Subjects:
image analysis
multiplex immunofluorescence
opal methodology
Online Access:https://doi.org/10.1002/1878-0261.12764

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