Malian adults maintain serologic responses to virulent PfEMP1s amid seasonal patterns of fluctuation
Abstract Plasmodium falciparum erythrocyte membrane protein-1s (PfEMP1s), diverse malaria proteins expressed on the infected erythrocyte surface, play an important role in pathogenesis, mediating adhesion to host vascular endothelium. Antibodies to particular non-CD36-binding PfEMP1s are associated...
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2021-07-01
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doaj-ddb8e7ed5a5b4e8b9edbd511c784d4972021-07-18T11:26:03ZengNature Publishing GroupScientific Reports2045-23222021-07-0111111410.1038/s41598-021-92974-7Malian adults maintain serologic responses to virulent PfEMP1s amid seasonal patterns of fluctuationNoah T. Ventimiglia0Emily M. Stucke1Drissa Coulibaly2Andrea A. Berry3Kirsten E. Lyke4Matthew B. Laurens5Jason A. Bailey6Matthew Adams7Amadou Niangaly8Abdoulaye K. Kone9Shannon Takala-Harrison10Bourema Kouriba11Ogobara K. Doumbo12Phillip L. Felgner13Christopher V. Plowe14Mahamadou A. Thera15Mark A. Travassos16University of Maryland School of MedicineUniversity of Maryland School of MedicineUniversity of Sciences, Techniques and TechnologiesUniversity of Maryland School of MedicineUniversity of Maryland School of MedicineUniversity of Maryland School of MedicineUniversity of Maryland School of MedicineUniversity of Maryland School of MedicineUniversity of Sciences, Techniques and TechnologiesUniversity of Sciences, Techniques and TechnologiesUniversity of Maryland School of MedicineUniversity of Sciences, Techniques and TechnologiesUniversity of Sciences, Techniques and TechnologiesUniversity of CaliforniaUniversity of Maryland School of MedicineUniversity of Sciences, Techniques and TechnologiesUniversity of Maryland School of MedicineAbstract Plasmodium falciparum erythrocyte membrane protein-1s (PfEMP1s), diverse malaria proteins expressed on the infected erythrocyte surface, play an important role in pathogenesis, mediating adhesion to host vascular endothelium. Antibodies to particular non-CD36-binding PfEMP1s are associated with protection against severe disease. We hypothesized that given lifelong P. falciparum exposure, Malian adults would have broad PfEMP1 serorecognition and high seroreactivity levels during follow-up, particularly to non-CD36-binding PfEMP1s such as those that attach to endothelial protein C receptor (EPCR) and intercellular adhesion molecule-1 (ICAM-1). Using a protein microarray, we determined serologic responses to 166 reference PfEMP1 fragments during a dry and subsequent malaria transmission season in Malian adults. Malian adult sera had PfEMP1 serologic responses throughout the year, with decreased reactivity to a small subset of PfEMP1 fragments during the dry season and increases in reactivity to a different subset of PfEMP1 fragments during the subsequent peak malaria transmission season, especially for intracellular PfEMP1 domains. For some individuals, PfEMP1 serologic responses increased after the dry season, suggesting antigenic switching during asymptomatic infection. Adults were more likely to experience variable serorecognition of CD36-binding PfEMP1s than non-CD36-binding PfEMP1s that bind EPCR or ICAM-1, which remained serorecognized throughout the year. Sustained seroreactivity to non-CD36-binding PfEMP1s throughout adulthood amid seasonal fluctuation patterns may reflect underlying protective severe malaria immunity and merits further investigation.https://doi.org/10.1038/s41598-021-92974-7 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Noah T. Ventimiglia Emily M. Stucke Drissa Coulibaly Andrea A. Berry Kirsten E. Lyke Matthew B. Laurens Jason A. Bailey Matthew Adams Amadou Niangaly Abdoulaye K. Kone Shannon Takala-Harrison Bourema Kouriba Ogobara K. Doumbo Phillip L. Felgner Christopher V. Plowe Mahamadou A. Thera Mark A. Travassos |
spellingShingle |
Noah T. Ventimiglia Emily M. Stucke Drissa Coulibaly Andrea A. Berry Kirsten E. Lyke Matthew B. Laurens Jason A. Bailey Matthew Adams Amadou Niangaly Abdoulaye K. Kone Shannon Takala-Harrison Bourema Kouriba Ogobara K. Doumbo Phillip L. Felgner Christopher V. Plowe Mahamadou A. Thera Mark A. Travassos Malian adults maintain serologic responses to virulent PfEMP1s amid seasonal patterns of fluctuation Scientific Reports |
author_facet |
Noah T. Ventimiglia Emily M. Stucke Drissa Coulibaly Andrea A. Berry Kirsten E. Lyke Matthew B. Laurens Jason A. Bailey Matthew Adams Amadou Niangaly Abdoulaye K. Kone Shannon Takala-Harrison Bourema Kouriba Ogobara K. Doumbo Phillip L. Felgner Christopher V. Plowe Mahamadou A. Thera Mark A. Travassos |
author_sort |
Noah T. Ventimiglia |
title |
Malian adults maintain serologic responses to virulent PfEMP1s amid seasonal patterns of fluctuation |
title_short |
Malian adults maintain serologic responses to virulent PfEMP1s amid seasonal patterns of fluctuation |
title_full |
Malian adults maintain serologic responses to virulent PfEMP1s amid seasonal patterns of fluctuation |
title_fullStr |
Malian adults maintain serologic responses to virulent PfEMP1s amid seasonal patterns of fluctuation |
title_full_unstemmed |
Malian adults maintain serologic responses to virulent PfEMP1s amid seasonal patterns of fluctuation |
title_sort |
malian adults maintain serologic responses to virulent pfemp1s amid seasonal patterns of fluctuation |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2021-07-01 |
description |
Abstract Plasmodium falciparum erythrocyte membrane protein-1s (PfEMP1s), diverse malaria proteins expressed on the infected erythrocyte surface, play an important role in pathogenesis, mediating adhesion to host vascular endothelium. Antibodies to particular non-CD36-binding PfEMP1s are associated with protection against severe disease. We hypothesized that given lifelong P. falciparum exposure, Malian adults would have broad PfEMP1 serorecognition and high seroreactivity levels during follow-up, particularly to non-CD36-binding PfEMP1s such as those that attach to endothelial protein C receptor (EPCR) and intercellular adhesion molecule-1 (ICAM-1). Using a protein microarray, we determined serologic responses to 166 reference PfEMP1 fragments during a dry and subsequent malaria transmission season in Malian adults. Malian adult sera had PfEMP1 serologic responses throughout the year, with decreased reactivity to a small subset of PfEMP1 fragments during the dry season and increases in reactivity to a different subset of PfEMP1 fragments during the subsequent peak malaria transmission season, especially for intracellular PfEMP1 domains. For some individuals, PfEMP1 serologic responses increased after the dry season, suggesting antigenic switching during asymptomatic infection. Adults were more likely to experience variable serorecognition of CD36-binding PfEMP1s than non-CD36-binding PfEMP1s that bind EPCR or ICAM-1, which remained serorecognized throughout the year. Sustained seroreactivity to non-CD36-binding PfEMP1s throughout adulthood amid seasonal fluctuation patterns may reflect underlying protective severe malaria immunity and merits further investigation. |
url |
https://doi.org/10.1038/s41598-021-92974-7 |
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