Functional Characterization of the Arabidopsis Ammonium Transporter AtAMT1;3 With the Emphasis on Structural Determinants of Substrate Binding and Permeation Properties

Functional Characterization of the Arabidopsis Ammonium Transporter AtAMT1;3 With the Emphasis on Structural Determinants of Substrate Binding and Permeation Properties

AtAMT1;3 is a major contributor to high-affinity ammonium uptake in Arabidopsis roots. Using a stable electrophysiological recording strategy, we demonstrate in Xenopus laevis oocytes that AtAMT1;3 functions as a typical high-affinity NH4+ uniporter independent of protons and Ca2+. The findings that...

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Bibliographic Details
Main Authors: Dong-Li Hao, Shun-Ying Yang, Shu-Xia Liu, Jin-Yan Zhou, Ya-Nan Huang, Anne-Aliénor Véry, Hervé Sentenac, Yan-Hua Su
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-05-01
Series:Frontiers in Plant Science
Subjects:
AtAMT1;3
electrophysiological assessment
point mutation
substrate binding
transport mechanism
homology modeling
Online Access:https://www.frontiersin.org/article/10.3389/fpls.2020.00571/full

Internet

https://www.frontiersin.org/article/10.3389/fpls.2020.00571/full

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