High throughput automated allele frequency estimation by pyrosequencing.

Pyrosequencing is a DNA sequencing method based on the principle of sequencing-by-synthesis and pyrophosphate detection through a series of enzymatic reactions. This bioluminometric, real-time DNA sequencing technique offers unique applications that are cost-effective and user-friendly. In this stud...

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Bibliographic Details
Main Authors: Julie Doostzadeh, Shadi Shokralla, Farnaz Absalan, Roxana Jalili, Sharareh Mohandessi, James W Langston, Ronald W Davis, Mostafa Ronaghi, Baback Gharizadeh
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2008-07-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC2442187?pdf=render