Multicenter Comparative Study of Six <i>Cryptosporidium parvum</i> DNA Extraction Protocols Including Mechanical Pretreatment from Stool Samples

Multicenter Comparative Study of Six <i>Cryptosporidium parvum</i> DNA Extraction Protocols Including Mechanical Pretreatment from Stool Samples

Background: Nowadays, many commercial kits allow the detection of <i>Cryptosporidium</i> sp. in stool samples after deoxyribonucleic acid (DNA) extraction. Protocols of stool pretreatment have been proposed to optimize oocysts’ DNA extraction. Among them, mechanical grinding was reported...

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Bibliographic Details
Main Authors: Nicolas Valeix, Damien Costa, Louise Basmaciyan, Stéphane Valot, Anne Vincent, Romy Razakandrainibe, Florence Robert-Gangneux, Céline Nourrisson, Bruno Pereira, Emilie Fréalle, Philippe Poirier, Loic Favennec, Frederic Dalle
Format: Article
Language:English
Published: MDPI AG 2020-09-01
Series:Microorganisms
Subjects:
<i>Cryptosporidium parvum</i>
grinding
DNA extraction
stool samples
real-time PCR
molecular diagnosis
Online Access:https://www.mdpi.com/2076-2607/8/9/1450

Internet

https://www.mdpi.com/2076-2607/8/9/1450

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