A comparative analysis of library prep approaches for sequencing low input translatome samples

A comparative analysis of library prep approaches for sequencing low input translatome samples

Abstract Background Cell type-specific ribosome-pulldown has become an increasingly popular method for analysis of gene expression. It allows for expression analysis from intact tissues and monitoring of protein synthesis in vivo. However, while its utility has been assessed, technical aspects relat...

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Bibliographic Details
Main Authors: Yang Song, Beatrice Milon, Sandra Ott, Xuechu Zhao, Lisa Sadzewicz, Amol Shetty, Erich T. Boger, Luke J. Tallon, Robert J. Morell, Anup Mahurkar, Ronna Hertzano
Format: Article
Language:English
Published: BMC 2018-09-01
Series:BMC Genomics
Subjects:
RiboTag
Library preparation kits
Low-input RNA-seq
RNA-seq
Coverage bias
Online Access:http://link.springer.com/article/10.1186/s12864-018-5066-2

Internet

http://link.springer.com/article/10.1186/s12864-018-5066-2

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