Robust adaptive optics for localization microscopy deep in complex tissue

It is difficult to apply SMLM to complex biological tissues. Here the authors report REALM, Robust and Effective Adaptive Optics in Localisation Microscopy, to improve SMLM in tissue and use this to resolve the organisation of spectrin in the axon initial segment in brain tissue.

Bibliographic Details
Main Authors: Marijn E. Siemons, Naomi A. K. Hanemaaijer, Maarten H. P. Kole, Lukas C. Kapitein
Format: Article
Language:English
Published: Nature Publishing Group 2021-06-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-021-23647-2