DMSO-Free Programmed Cryopreservation of Fully Dissociated and Adherent Human Induced Pluripotent Stem Cells

Three modes for cryopreservation (CP) of human iPSC cells have been compared: STD: standard CP of small clumps with 10% of CPA in cryovials, ACC: dissociation of the cells with Accutase and freezing in cryovials, and PLT: programmed freezing of adherent cells in plastic multiwell dishes in a program...

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Bibliographic Details
Main Authors: Igor I. Katkov, Natalia G. Kan, Flavio Cimadamore, Brandon Nelson, Evan Y. Snyder, Alexey V. Terskikh
Format: Article
Language:English
Published: Hindawi Limited 2011-01-01
Series:Stem Cells International
Online Access:http://dx.doi.org/10.4061/2011/981606