SNAP-, CLIP- and Halo-tag labelling of budding yeast cells.

Fluorescence microscopy of the localization and the spatial and temporal dynamics of specifically labelled proteins is an indispensable tool in cell biology. Besides fluorescent proteins as tags, tag-mediated labelling utilizing self-labelling proteins as the SNAP-, CLIP-, or the Halo-tag are widely...

Full description

Bibliographic Details
Main Authors: Franziska Stagge, Gyuzel Y Mitronova, Vladimir N Belov, Christian A Wurm, Stefan Jakobs
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3808294?pdf=render