Effective control of large deletions after double-strand breaks by homology-directed repair and dsODN insertion

Effective control of large deletions after double-strand breaks by homology-directed repair and dsODN insertion

Abstract Background After repairing double-strand breaks (DSBs) caused by CRISPR-Cas9 cleavage, genomic damage, such as large deletions, may have pathogenic consequences. Results We show that large deletions are ubiquitous but are dependent on editing sites and cell types. Human primary T cells disp...

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Bibliographic Details
Main Authors: Wei Wen, Zi-Jun Quan, Si-Ang Li, Zhi-Xue Yang, Ya-Wen Fu, Feng Zhang, Guo-Hua Li, Mei Zhao, Meng-Di Yin, Jing Xu, Jian-Ping Zhang, Tao Cheng, Xiao-Bing Zhang
Format: Article
Language:English
Published: BMC 2021-08-01
Series:Genome Biology
Subjects:
CRISPR-Cas9
Genome editing
Large fragment deletions
Nanopore sequencing
Homology-directed repair (HDR)
Non-homologous end joining (NHEJ)
Online Access:https://doi.org/10.1186/s13059-021-02462-4

Internet

https://doi.org/10.1186/s13059-021-02462-4

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