A gene-specific T2A-GAL4 library for Drosophila

A gene-specific T2A-GAL4 library for Drosophila

We generated a library of ~1000 Drosophila stocks in which we inserted a construct in the intron of genes allowing expression of GAL4 under control of endogenous promoters while arresting transcription with a polyadenylation signal 3’ of the GAL4. This allows numerous applications. First, ~90% of in...

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Bibliographic Details
Main Authors: Pei-Tseng Lee, Jonathan Zirin, Oguz Kanca, Wen-Wen Lin, Karen L Schulze, David Li-Kroeger, Rong Tao, Colby Devereaux, Yanhui Hu, Verena Chung, Ying Fang, Yuchun He, Hongling Pan, Ming Ge, Zhongyuan Zuo, Benjamin E Housden, Stephanie E Mohr, Shinya Yamamoto, Robert W Levis, Allan C Spradling, Norbert Perrimon, Hugo J Bellen
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2018-03-01
Series:eLife
Subjects:
MiMIC
CRIMIC
GAL4/UAS
complementation
cassette excision
loss of function
Online Access:https://elifesciences.org/articles/35574

Internet

https://elifesciences.org/articles/35574

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