A user-friendly platform for yeast two-hybrid library screening using next generation sequencing.

Yeast two-hybrid (Y2H) is a well-established genetics-based system that uses yeast to selectively display binary protein-protein interactions (PPIs). To meet the current need to unravel complex PPI networks, several adaptations have been made to establish medium- to high-throughput Y2H screening pla...

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Bibliographic Details
Main Authors: Marie-Laure Erffelinck, Bianca Ribeiro, Maria Perassolo, Laurens Pauwels, Jacob Pollier, Veronique Storme, Alain Goossens
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2018-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0201270