Essential role of polymerases for assay performance – Impact of polymerase replacement in a well-established assay

The quantitative real-time polymerase chain reaction (qPCR) is one of the most commonly molecular methods used today. It is central to numerous assays that have since been developed and described around its optimization. The Listeria monocytogenes prfA qPCR assay has been studied in great detail and...

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Bibliographic Details
Main Authors: Anna Kristina Witte, Romana Sickha, Patrick Mester, Susanne Fister, Dagmar Schoder, Peter Rossmanith
Format: Article
Language:English
Published: Elsevier 2018-12-01
Series:Biomolecular Detection and Quantification
Online Access:http://www.sciencedirect.com/science/article/pii/S2214753518300093