Establishment of lipofection for studying miRNA function in human adipocytes.

miRNA dysregulation has recently been linked to human obesity and its related complications such as type 2 diabetes. In order to study miRNA function in human adipocytes, we aimed for the modulation of mature miRNA concentration in these cells. Adipocytes, however, tend to be resistant to transfecti...

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Bibliographic Details
Main Authors: Eveliina Enlund, Simon Fischer, René Handrick, Kerstin Otte, Klaus-Michael Debatin, Martin Wabitsch, Pamela Fischer-Posovszky
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4029777?pdf=render