Immunofluorescent staining of cancer spheroids and fine-needle aspiration-derived organoids

Summary: Our organoid generation technique has allowed for the development of downstream organoid applications. Here, we detail an accessible, straightforward protocol for immunofluorescent staining and imaging of thyroid cancer organoids, particularly those with tumor de-differentiation. Immunofluo...

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Bibliographic Details
Main Authors: Kensey N. Bergdorf, Courtney J. Phifer, Matthew E. Bechard, Mason A. Lee, Oliver G. McDonald, Ethan Lee, Vivian L. Weiss
Format: Article
Language:English
Published: Elsevier 2021-06-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721002859