Loop-mediated isothermal amplification (LAMP) reaction as viable PCR substitute for diagnostic applications: a comparative analysis study of LAMP, conventional PCR, nested PCR (nPCR) and real-time PCR (qPCR) based on Entamoeba histolytica DNA derived from faecal sample

Loop-mediated isothermal amplification (LAMP) reaction as viable PCR substitute for diagnostic applications: a comparative analysis study of LAMP, conventional PCR, nested PCR (nPCR) and real-time PCR (qPCR) based on Entamoeba histolytica DNA derived from faecal sample

Abstract Background This study reports the analytical sensitivity and specificity of a Loop-mediated isothermal amplification (LAMP) and compares its amplification performance with conventional PCR, nested PCR (nPCR) and real-time PCR (qPCR). All the assays demonstrated in this study were developed...

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Bibliographic Details
Main Authors: Phiaw Chong Foo, A. B. Nurul Najian, Nuramin A. Muhamad, Mariana Ahamad, Maizan Mohamed, Chan Yean Yean, Boon Huat Lim
Format: Article
Language:English
Published: BMC 2020-06-01
Series:BMC Biotechnology
Subjects:
Loop-mediated isothermal amplification
Nested PCR
Real-time PCR
Lateral flow dipstick
Calcein-manganese visualization
LAMP analytical sensitivity
Online Access:http://link.springer.com/article/10.1186/s12896-020-00629-8

Internet

http://link.springer.com/article/10.1186/s12896-020-00629-8

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