Loop-mediated isothermal amplification (LAMP) reaction as viable PCR substitute for diagnostic applications: a comparative analysis study of LAMP, conventional PCR, nested PCR (nPCR) and real-time PCR (qPCR) based on Entamoeba histolytica DNA derived from faecal sample
Abstract Background This study reports the analytical sensitivity and specificity of a Loop-mediated isothermal amplification (LAMP) and compares its amplification performance with conventional PCR, nested PCR (nPCR) and real-time PCR (qPCR). All the assays demonstrated in this study were developed...
Main Authors: | Phiaw Chong Foo, A. B. Nurul Najian, Nuramin A. Muhamad, Mariana Ahamad, Maizan Mohamed, Chan Yean Yean, Boon Huat Lim |
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Format: | Article |
Language: | English |
Published: |
BMC
2020-06-01
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Series: | BMC Biotechnology |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s12896-020-00629-8 |
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