Antifungal activity of two oxadiazole compounds for the paracoccidioidomycosis treatment.
Paracoccidioidomycosis (PCM) is a neglected disease present in Latin America with difficulty in treatment and occurrence of serious sequelae. Thus, the development of alternative therapies is imperative. In the current work, two oxadiazole compounds (LMM5 and LMM11) presented fungicidal activity aga...
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doaj-e07e212d7a984a278f4ae13253c47b982021-04-21T23:54:24ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352019-06-01136e000744110.1371/journal.pntd.0007441Antifungal activity of two oxadiazole compounds for the paracoccidioidomycosis treatment.Franciele Abigail Vilugron Rodrigues-VendraminiDaniella Renata FariaGlaucia Sayuri AritaIsis Regina Grenier CapociKarina Mayumi SakitaSilvana Martins Caparroz-AssefTania Cristina Alexandrino BeckerPatrícia de Souza Bonfim-MendonçaMaria Sueli FelipeTerezinha Inez Estivalet SvidzinskiBernard MaigretÉrika Seki KioshimaParacoccidioidomycosis (PCM) is a neglected disease present in Latin America with difficulty in treatment and occurrence of serious sequelae. Thus, the development of alternative therapies is imperative. In the current work, two oxadiazole compounds (LMM5 and LMM11) presented fungicidal activity against Paracoccidioides spp. The minimum inhibitory and fungicidal concentration values ranged from 1 to 32 μg/mL, and a synergic effect was observed for both compounds when combined with Amphotericin B. LMM5 and LMM11 were able to reduce CFU counts (≥2 log10) on the 5th and 7th days of time-kill curve, respectively. The fungicide effect was confirmed by fluorescence microscopy (FUN-1/FUN-2). The hippocratic screening and biochemical analysis were performed in Balb/c male mice that received a high dose of each compound, and the compounds showed no in vivo toxicity. The treatment of experimental PCM with the new oxadiazoles led to significant reduction in CFU (≥1 log10). Histopathological analysis of the groups treated exhibited control of inflammation, as well as preserved lung areas. These findings suggest that LMM5 and LMM11 are promising hits structures, opening the door for implementing new PCM therapies.https://doi.org/10.1371/journal.pntd.0007441 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Franciele Abigail Vilugron Rodrigues-Vendramini Daniella Renata Faria Glaucia Sayuri Arita Isis Regina Grenier Capoci Karina Mayumi Sakita Silvana Martins Caparroz-Assef Tania Cristina Alexandrino Becker Patrícia de Souza Bonfim-Mendonça Maria Sueli Felipe Terezinha Inez Estivalet Svidzinski Bernard Maigret Érika Seki Kioshima |
spellingShingle |
Franciele Abigail Vilugron Rodrigues-Vendramini Daniella Renata Faria Glaucia Sayuri Arita Isis Regina Grenier Capoci Karina Mayumi Sakita Silvana Martins Caparroz-Assef Tania Cristina Alexandrino Becker Patrícia de Souza Bonfim-Mendonça Maria Sueli Felipe Terezinha Inez Estivalet Svidzinski Bernard Maigret Érika Seki Kioshima Antifungal activity of two oxadiazole compounds for the paracoccidioidomycosis treatment. PLoS Neglected Tropical Diseases |
author_facet |
Franciele Abigail Vilugron Rodrigues-Vendramini Daniella Renata Faria Glaucia Sayuri Arita Isis Regina Grenier Capoci Karina Mayumi Sakita Silvana Martins Caparroz-Assef Tania Cristina Alexandrino Becker Patrícia de Souza Bonfim-Mendonça Maria Sueli Felipe Terezinha Inez Estivalet Svidzinski Bernard Maigret Érika Seki Kioshima |
author_sort |
Franciele Abigail Vilugron Rodrigues-Vendramini |
title |
Antifungal activity of two oxadiazole compounds for the paracoccidioidomycosis treatment. |
title_short |
Antifungal activity of two oxadiazole compounds for the paracoccidioidomycosis treatment. |
title_full |
Antifungal activity of two oxadiazole compounds for the paracoccidioidomycosis treatment. |
title_fullStr |
Antifungal activity of two oxadiazole compounds for the paracoccidioidomycosis treatment. |
title_full_unstemmed |
Antifungal activity of two oxadiazole compounds for the paracoccidioidomycosis treatment. |
title_sort |
antifungal activity of two oxadiazole compounds for the paracoccidioidomycosis treatment. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS Neglected Tropical Diseases |
issn |
1935-2727 1935-2735 |
publishDate |
2019-06-01 |
description |
Paracoccidioidomycosis (PCM) is a neglected disease present in Latin America with difficulty in treatment and occurrence of serious sequelae. Thus, the development of alternative therapies is imperative. In the current work, two oxadiazole compounds (LMM5 and LMM11) presented fungicidal activity against Paracoccidioides spp. The minimum inhibitory and fungicidal concentration values ranged from 1 to 32 μg/mL, and a synergic effect was observed for both compounds when combined with Amphotericin B. LMM5 and LMM11 were able to reduce CFU counts (≥2 log10) on the 5th and 7th days of time-kill curve, respectively. The fungicide effect was confirmed by fluorescence microscopy (FUN-1/FUN-2). The hippocratic screening and biochemical analysis were performed in Balb/c male mice that received a high dose of each compound, and the compounds showed no in vivo toxicity. The treatment of experimental PCM with the new oxadiazoles led to significant reduction in CFU (≥1 log10). Histopathological analysis of the groups treated exhibited control of inflammation, as well as preserved lung areas. These findings suggest that LMM5 and LMM11 are promising hits structures, opening the door for implementing new PCM therapies. |
url |
https://doi.org/10.1371/journal.pntd.0007441 |
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