Improved CRISPR/Cas9 gene editing in primary human myoblasts using low confluency cultures on Matrigel

Improved CRISPR/Cas9 gene editing in primary human myoblasts using low confluency cultures on Matrigel

Abstract Background CRISPR/Cas9 is an invaluable tool for studying cell biology and the development of molecular therapies. However, delivery of CRISPR/Cas9 components into some cell types remains a major hurdle. Primary human myoblasts are a valuable cell model for muscle studies, but are notorious...

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Bibliographic Details
Main Authors: Hayley Goullée, Rhonda L. Taylor, Alistair R. R. Forrest, Nigel G. Laing, Gianina Ravenscroft, Joshua S. Clayton
Format: Article
Language:English
Published: BMC 2021-09-01
Series:Skeletal Muscle
Subjects:
CRISPR
Gene editing efficiency
Primary human myoblasts
Matrigel
Confluency
Online Access:https://doi.org/10.1186/s13395-021-00278-1

Internet

https://doi.org/10.1186/s13395-021-00278-1

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