FoldAffinity: binding affinities from nDSF experiments

Abstract Differential scanning fluorimetry (DSF) using the inherent fluorescence of proteins (nDSF) is a popular technique to evaluate thermal protein stability in different conditions (e.g. buffer, pH). In many cases, ligand binding increases thermal stability of a protein and often this can be det...

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Bibliographic Details
Main Authors: Stephan Niebling, Osvaldo Burastero, Jérôme Bürgi, Christian Günther, Lucas A. Defelipe, Simon Sander, Ellen Gattkowski, Raghavendra Anjanappa, Matthias Wilmanns, Sebastian Springer, Henning Tidow, María García-Alai
Format: Article
Language:English
Published: Nature Publishing Group 2021-05-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-021-88985-z