CLEC4M慢病毒载体的构建及其在K562细胞中的表达
ObjectiveTo construct the lentiviral vector encoding CLEC4M and prepare K-562 cells with stable overexpression of CLEC4M. MethodsThe gene sequence of normal CLEC4M was cloned by RT-PCR and then inserted into GV166 vector to construct GV166-CLEC4M lentiviral expression vector, and then lentiviral pac...
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| Format: | Article |
| Language: | zho |
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Editorial Department of Journal of Clinical Hepatology
2014-06-01
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| Series: | Linchuang Gandanbing Zazhi |
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| Online Access: | http://www.lcgdbzz.org/qk_content.asp?id=5815&ClassID=42414109 |