BRCA1 Accelerates CtIP-Mediated DNA-End Resection

BRCA1 Accelerates CtIP-Mediated DNA-End Resection

DNA-end resection is a highly regulated and critical step in the response and repair of DNA double-strand breaks. In higher eukaryotes, CtIP regulates resection by integrating cellular signals via its posttranslational modifications and protein-protein interactions, including cell-cycle-controlled i...

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Main Authors: Andrés Cruz-García, Ana López-Saavedra, Pablo Huertas
Format: Article
Language:English
Published: Elsevier 2014-10-01
Series:Cell Reports
Online Access:http://www.sciencedirect.com/science/article/pii/S2211124714007682
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spelling doaj-e14cb73f6e9a44a7b1a865002f8a757d2020-11-24T21:47:22ZengElsevierCell Reports2211-12472014-10-019245145910.1016/j.celrep.2014.08.076BRCA1 Accelerates CtIP-Mediated DNA-End ResectionAndrés Cruz-García0Ana López-Saavedra1Pablo Huertas2Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER), 41092 Sevilla, SpainCentro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER), 41092 Sevilla, SpainCentro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER), 41092 Sevilla, SpainDNA-end resection is a highly regulated and critical step in the response and repair of DNA double-strand breaks. In higher eukaryotes, CtIP regulates resection by integrating cellular signals via its posttranslational modifications and protein-protein interactions, including cell-cycle-controlled interaction with BRCA1. The role of BRCA1 in DNA-end resection is not clear. Here, we develop an assay to study DNA resection in higher eukaryotes at high resolution. We demonstrate that the BRCA1-CtIP interaction, albeit not essential for resection, modulates the speed at which this process takes place.http://www.sciencedirect.com/science/article/pii/S2211124714007682
collection DOAJ
language English
format Article
sources DOAJ
author Andrés Cruz-García
Ana López-Saavedra
Pablo Huertas
spellingShingle Andrés Cruz-García
Ana López-Saavedra
Pablo Huertas
BRCA1 Accelerates CtIP-Mediated DNA-End Resection
Cell Reports
author_facet Andrés Cruz-García
Ana López-Saavedra
Pablo Huertas
author_sort Andrés Cruz-García
title BRCA1 Accelerates CtIP-Mediated DNA-End Resection
title_short BRCA1 Accelerates CtIP-Mediated DNA-End Resection
title_full BRCA1 Accelerates CtIP-Mediated DNA-End Resection
title_fullStr BRCA1 Accelerates CtIP-Mediated DNA-End Resection
title_full_unstemmed BRCA1 Accelerates CtIP-Mediated DNA-End Resection
title_sort brca1 accelerates ctip-mediated dna-end resection
publisher Elsevier
series Cell Reports
issn 2211-1247
publishDate 2014-10-01
description DNA-end resection is a highly regulated and critical step in the response and repair of DNA double-strand breaks. In higher eukaryotes, CtIP regulates resection by integrating cellular signals via its posttranslational modifications and protein-protein interactions, including cell-cycle-controlled interaction with BRCA1. The role of BRCA1 in DNA-end resection is not clear. Here, we develop an assay to study DNA resection in higher eukaryotes at high resolution. We demonstrate that the BRCA1-CtIP interaction, albeit not essential for resection, modulates the speed at which this process takes place.
url http://www.sciencedirect.com/science/article/pii/S2211124714007682
work_keys_str_mv AT andrescruzgarcia brca1acceleratesctipmediateddnaendresection
AT analopezsaavedra brca1acceleratesctipmediateddnaendresection
AT pablohuertas brca1acceleratesctipmediateddnaendresection
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