In vitro synthesis of phospholipids with yeast phospholipase B, a phospholipid deacylating enzyme

The gene encoding the Saccharomyces cerevisiae phospholipid deacylation enzyme, phospholipase B (ScPLB1), was successfully expressed in E. coli. The enzyme (Scplb1p) was engineered to have a histidine-tag at the C-terminal end and was purified by metal (Ni) affinity chromatography. Enzymatic propert...

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Bibliographic Details
Main Authors: Yasuo Watanabe, Itsuki Kobayashi, Takanori Ohnaka, Seiya Watanabe
Format: Article
Language:English
Published: Elsevier 2018-06-01
Series:Biotechnology Reports
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2215017X17303107