A novel SH2 recognition mechanism recruits Spt6 to the doubly phosphorylated RNA polymerase II linker at sites of transcription

A novel SH2 recognition mechanism recruits Spt6 to the doubly phosphorylated RNA polymerase II linker at sites of transcription

We determined that the tandem SH2 domain of S. cerevisiae Spt6 binds the linker region of the RNA polymerase II subunit Rpb1 rather than the expected sites in its heptad repeat domain. The 4 nM binding affinity requires phosphorylation at Rpb1 S1493 and either T1471 or Y1473. Crystal structures show...

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Bibliographic Details
Main Authors: Matthew A Sdano, James M Fulcher, Sowmiya Palani, Mahesh B Chandrasekharan, Timothy J Parnell, Frank G Whitby, Tim Formosa, Christopher P Hill
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2017-08-01
Series:eLife
Subjects:
Spt6
transcription
phosphorylation
RNA polymerase II
histone chaperone
SH2 domain
Online Access:https://elifesciences.org/articles/28723

Internet

https://elifesciences.org/articles/28723

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