Ultrasensitive nucleic acid detection based on phosphorothioated hairpin-assisted isothermal amplification

Abstract Herein, we describe a phosphorothioated hairpin-assisted isothermal amplification (PHAmp) method for detection of a target nucleic acid. The hairpin probe (HP) is designed to contain a 5′ phosphorothioate (PS)-modified overhang, a target recognition site, and a 3′ self-priming (SP) region....

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Bibliographic Details
Main Authors: Yujin Jung, Jayeon Song, Hyun Gyu Park
Format: Article
Language:English
Published: Nature Publishing Group 2021-04-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-021-87948-8