Application of nonsense-mediated primer exclusion (NOPE) for preparation of unique molecular barcoded libraries

Application of nonsense-mediated primer exclusion (NOPE) for preparation of unique molecular barcoded libraries

Abstract Background Recently we proposed efficient method to exclude undesirable primers at any stage of amplification reaction, here termed NOPE (NOnsense-mediated Primer Exclusion). According to this method, added oligonucleotide overlapping with the 3′-end of unwanted amplification primer (NOPE o...

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Bibliographic Details
Main Authors: Dmitriy A. Shagin, Maria A. Turchaninova, Irina A. Shagina, Mikhail Shugay, Andrew R. Zaretsky, Olga I. Zueva, Dmitriy A. Bolotin, Sergey Lukyanov, Dmitriy M. Chudakov
Format: Article
Language:English
Published: BMC 2017-06-01
Series:BMC Genomics
Subjects:
High-throughput sequencing
Unique molecular identifiers
Targeted resequencing
PCR
Online Access:http://link.springer.com/article/10.1186/s12864-017-3815-2

Internet

http://link.springer.com/article/10.1186/s12864-017-3815-2

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