Efficient dual-negative selection for bacterial genome editing

Efficient dual-negative selection for bacterial genome editing

Abstract Background Gene editing is key for elucidating gene function. Traditional methods, such as consecutive single-crossovers, have been widely used to modify bacterial genomes. However, cumbersome cloning and limited efficiency of negative selection often make this method slower than other meth...

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Bibliographic Details
Main Authors: Francesca Romana Cianfanelli, Olivier Cunrath, Dirk Bumann
Format: Article
Language:English
Published: BMC 2020-05-01
Series:BMC Microbiology
Subjects:
Salmonella
Homologous recombination
Mutagenesis
Gene manipulation
MDR
Online Access:http://link.springer.com/article/10.1186/s12866-020-01819-2

Internet

http://link.springer.com/article/10.1186/s12866-020-01819-2

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