Interactions of pancreatic lipase with bile salts and dodecyl sulfate

Interactions of pancreatic lipase with bile salts and dodecyl sulfate

Sodium dodecyl sulfate (SDS) binds to pancreatic lipase in a cooperative manner up to about 200 moles/mole of protein. This binding in a rapid and irreversible inactivation of lipase. Bile salts under certain conditions prevent SDS inactivation of lipase when both detergents are present together. Un...

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Bibliographic Details
Main Authors: B Borgstrom, J Donnér
Format: Article
Language:English
Published: Elsevier 1976-09-01
Series:Journal of Lipid Research
Subjects:
dodecanoyl taurate
equilibrium dialysis
Online Access:http://www.sciencedirect.com/science/article/pii/S002222752041747X
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spelling doaj-e1fff44393b745899fd6f552303bcae12021-04-24T05:54:14ZengElsevierJournal of Lipid Research0022-22751976-09-01175491497Interactions of pancreatic lipase with bile salts and dodecyl sulfateB Borgstrom0J Donnér1Department of Physiological Chemistry, University of Lund, Lund, SwedenDepartment of Physiological Chemistry, University of Lund, Lund, SwedenSodium dodecyl sulfate (SDS) binds to pancreatic lipase in a cooperative manner up to about 200 moles/mole of protein. This binding in a rapid and irreversible inactivation of lipase. Bile salts under certain conditions prevent SDS inactivation of lipase when both detergents are present together. Under these conditions bile salts also prevent the binding of SDS to lipase. This effect is parallel to and probably mediated by a decrease in the SDS monomer concentration as the result of the formation of mixed bile salt–SDS micelles.http://www.sciencedirect.com/science/article/pii/S002222752041747Xdodecanoyl taurateequilibrium dialysis
collection DOAJ
language English
format Article
sources DOAJ
author B Borgstrom
J Donnér
spellingShingle B Borgstrom
J Donnér
Interactions of pancreatic lipase with bile salts and dodecyl sulfate
Journal of Lipid Research
dodecanoyl taurate
equilibrium dialysis
author_facet B Borgstrom
J Donnér
author_sort B Borgstrom
title Interactions of pancreatic lipase with bile salts and dodecyl sulfate
title_short Interactions of pancreatic lipase with bile salts and dodecyl sulfate
title_full Interactions of pancreatic lipase with bile salts and dodecyl sulfate
title_fullStr Interactions of pancreatic lipase with bile salts and dodecyl sulfate
title_full_unstemmed Interactions of pancreatic lipase with bile salts and dodecyl sulfate
title_sort interactions of pancreatic lipase with bile salts and dodecyl sulfate
publisher Elsevier
series Journal of Lipid Research
issn 0022-2275
publishDate 1976-09-01
description Sodium dodecyl sulfate (SDS) binds to pancreatic lipase in a cooperative manner up to about 200 moles/mole of protein. This binding in a rapid and irreversible inactivation of lipase. Bile salts under certain conditions prevent SDS inactivation of lipase when both detergents are present together. Under these conditions bile salts also prevent the binding of SDS to lipase. This effect is parallel to and probably mediated by a decrease in the SDS monomer concentration as the result of the formation of mixed bile salt–SDS micelles.
topic dodecanoyl taurate
equilibrium dialysis
url http://www.sciencedirect.com/science/article/pii/S002222752041747X
work_keys_str_mv AT bborgstrom interactionsofpancreaticlipasewithbilesaltsanddodecylsulfate
AT jdonner interactionsofpancreaticlipasewithbilesaltsanddodecylsulfate
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