Crystal structures of the PH domains from Lbc family of RhoGEFs bound to activated RhoA GTPase
The Pleckstrin homology (PH) domains from the Lbc family of Rho Guanine Nucleotide Exchange Factors (Lbc RhoGEFs) interact with activated Rho family GTPases. All 7 Lbc RhoGEFs associate directly with activated Rho GTPases via their PH domains. However, the binding affinities between the PH domains a...
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doaj-e2acd39432b844e8a0410010041fd3672020-11-24T23:56:53ZengElsevierData in Brief2352-34092018-04-0117356362Crystal structures of the PH domains from Lbc family of RhoGEFs bound to activated RhoA GTPaseZhe Chen0Steven Gutowski1Paul C. Sternweis2Department of Biophysics, The University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390, United States; Corresponding author.Department of Pharmacology, The University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390, United StatesDepartment of Pharmacology, The University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390, United StatesThe Pleckstrin homology (PH) domains from the Lbc family of Rho Guanine Nucleotide Exchange Factors (Lbc RhoGEFs) interact with activated Rho family GTPases. All 7 Lbc RhoGEFs associate directly with activated Rho GTPases via their PH domains. However, the binding affinities between the PH domains and the GTPases vary greatly. Here we present two crystal structures at resolutions of 1.4 Å and 2.0 Å of RhoA complexed with the PH domain from p114RhoGEF (PDB access code 6BCB) and AKAP-LbcRhoGEF (PDB access code 6BCA), respectively. These high resolution structures, together with the earlier structures of PDZRhoGEF-PH·RhoA and p190RhoGEF-PH·RhoA complexes, identify a highly conserved interface between the PH domains from Lbc-RhoGEFs and activated Rho GTPases. This manuscript is related to the manuscript titled “Direct Regulation of p190RhoGEF by Activated Rho and Rac GTPases” published in the Journal of Structural Biology.http://www.sciencedirect.com/science/article/pii/S2352340918300271 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zhe Chen Steven Gutowski Paul C. Sternweis |
spellingShingle |
Zhe Chen Steven Gutowski Paul C. Sternweis Crystal structures of the PH domains from Lbc family of RhoGEFs bound to activated RhoA GTPase Data in Brief |
author_facet |
Zhe Chen Steven Gutowski Paul C. Sternweis |
author_sort |
Zhe Chen |
title |
Crystal structures of the PH domains from Lbc family of RhoGEFs bound to activated RhoA GTPase |
title_short |
Crystal structures of the PH domains from Lbc family of RhoGEFs bound to activated RhoA GTPase |
title_full |
Crystal structures of the PH domains from Lbc family of RhoGEFs bound to activated RhoA GTPase |
title_fullStr |
Crystal structures of the PH domains from Lbc family of RhoGEFs bound to activated RhoA GTPase |
title_full_unstemmed |
Crystal structures of the PH domains from Lbc family of RhoGEFs bound to activated RhoA GTPase |
title_sort |
crystal structures of the ph domains from lbc family of rhogefs bound to activated rhoa gtpase |
publisher |
Elsevier |
series |
Data in Brief |
issn |
2352-3409 |
publishDate |
2018-04-01 |
description |
The Pleckstrin homology (PH) domains from the Lbc family of Rho Guanine Nucleotide Exchange Factors (Lbc RhoGEFs) interact with activated Rho family GTPases. All 7 Lbc RhoGEFs associate directly with activated Rho GTPases via their PH domains. However, the binding affinities between the PH domains and the GTPases vary greatly. Here we present two crystal structures at resolutions of 1.4 Å and 2.0 Å of RhoA complexed with the PH domain from p114RhoGEF (PDB access code 6BCB) and AKAP-LbcRhoGEF (PDB access code 6BCA), respectively. These high resolution structures, together with the earlier structures of PDZRhoGEF-PH·RhoA and p190RhoGEF-PH·RhoA complexes, identify a highly conserved interface between the PH domains from Lbc-RhoGEFs and activated Rho GTPases. This manuscript is related to the manuscript titled “Direct Regulation of p190RhoGEF by Activated Rho and Rac GTPases” published in the Journal of Structural Biology. |
url |
http://www.sciencedirect.com/science/article/pii/S2352340918300271 |
work_keys_str_mv |
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