Rapid apolipoprotein E phenotyping by immunofixation in agarose
Conventional determination of apolipoprotein E isomorphs comprises ultracentrifugation of 1-5 ml serum, delipidation of very low density lipoproteins (VLDL), and isoelectric focusing (IEF) in polyacrylamide gels. In order to reduce the sample volume and to avoid nonspecific protein bands, immunoblot...
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
Elsevier
1991-05-01
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Series: | Journal of Lipid Research |
Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520420401 |