Rapid apolipoprotein E phenotyping by immunofixation in agarose

Conventional determination of apolipoprotein E isomorphs comprises ultracentrifugation of 1-5 ml serum, delipidation of very low density lipoproteins (VLDL), and isoelectric focusing (IEF) in polyacrylamide gels. In order to reduce the sample volume and to avoid nonspecific protein bands, immunoblot...

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Bibliographic Details
Main Authors: C Luley, MW Baumstark, H Wieland
Format: Article
Language:English
Published: Elsevier 1991-05-01
Series:Journal of Lipid Research
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520420401