Analytical and clinical evaluation of a heat shock SARS-CoV-2 detection method without RNA extraction for N and E genes RT-qPCR

Analytical and clinical evaluation of a heat shock SARS-CoV-2 detection method without RNA extraction for N and E genes RT-qPCR

Background: The COVID-19 pandemic has caused significant supply shortages worldwide for SARS-CoV-2 molecular diagnosis, like RNA extraction kits. Objective: The aim of our study was to evaluate the clinical performance and analytical sensitivity of a simple SARS-CoV-2 diagnosis protocol based on hea...

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Bibliographic Details
Main Authors: Alfredo Bruno, Domenica de Mora, Byron Freire-Paspuel, Angel S. Rodriguez, Maria Belen Paredes-Espinosa, Maritza Olmedo, Martha Sanchez, Jennifer Romero, Michelle Paez, Manuel Gonzalez, Alberto Orlando, Miguel Angel Garcia-Bereguiain
Format: Article
Language:English
Published: Elsevier 2021-08-01
Series:International Journal of Infectious Diseases
Subjects:
SARS-CoV-2
RT-qPCR
RNA extraction free
Heat shock
COVID-19
Online Access:http://www.sciencedirect.com/science/article/pii/S1201971221005269

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http://www.sciencedirect.com/science/article/pii/S1201971221005269

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