Aptamer-Driven Toxin Gene Delivery in U87 Model Glioblastoma Cells

Aptamer-Driven Toxin Gene Delivery in U87 Model Glioblastoma Cells

A novel suicide gene therapy approach was tested in U87 MG glioblastoma multiforme cells. A 26nt G-rich double-stranded DNA aptamer (AS1411) was integrated into a vector at the 5′ of a mammalian codon-optimized saporin gene, under CMV promoter. With this plasmid termed “APTSAP”, the gene encoding ri...

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Bibliographic Details
Main Authors: Luana di Leandro, Francesco Giansanti, Sabrina Mei, Sara Ponziani, Martina Colasante, Matteo Ardini, Francesco Angelucci, Giuseppina Pitari, Michele d’Angelo, Annamaria Cimini, Maria Serena Fabbrini, Rodolfo Ippoliti
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-04-01
Series:Frontiers in Pharmacology
Subjects:
glioblastoma
AS1411 aptamer
DNA-based gene delivery
saporin
nucleolin
Online Access:https://www.frontiersin.org/articles/10.3389/fphar.2021.588306/full

Internet

https://www.frontiersin.org/articles/10.3389/fphar.2021.588306/full

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