Rapid, Single-Cell Analysis and Discovery of Vectored mRNA Transfection In Vivo with a loxP-Flanked tdTomato Reporter Mouse

Rapid, Single-Cell Analysis and Discovery of Vectored mRNA Transfection In Vivo with a loxP-Flanked tdTomato Reporter Mouse

mRNA therapeutics hold promise for the treatment of diseases requiring intracellular protein expression and for use in genome editing systems, but mRNA must transfect the desired tissue and cell type to be efficacious. Nanoparticle vectors that deliver the mRNA are often evaluated using mRNA encodin...

Full description

Bibliographic Details
Main Authors: Kevin J. Kauffman, Matthias A. Oberli, J. Robert Dorkin, Juan E. Hurtado, James C. Kaczmarek, Shivani Bhadani, Jeff Wyckoff, Robert Langer, Ana Jaklenec, Daniel G. Anderson
Format: Article
Language:English
Published: Elsevier 2018-03-01
Series:Molecular Therapy: Nucleic Acids
Subjects:
mRNA
nanoparticles
reporter mouse
single-cell analysis
flow cytometry
Online Access:http://www.sciencedirect.com/science/article/pii/S2162253117302895

Internet

http://www.sciencedirect.com/science/article/pii/S2162253117302895

Similar Items