Automated mass spectrometry imaging of over 2000 proteins from tissue sections at 100-μm spatial resolution
Imaging mass spectrometry is a powerful emerging tool for mapping the spatial distribution of biomolecules across tissue surfaces. Here the authors showcase an automated technology for deep proteome imaging that utilizes ultrasensitive microfluidics and a mass spectrometry workflow to analyze tissue...
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| Format: | Article |
| Language: | English |
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Nature Publishing Group
2020-01-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-019-13858-z |
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Article |
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doaj-e3b5a3a1d7b147dd83097e22370e56dc2021-05-11T08:31:43ZengNature Publishing GroupNature Communications2041-17232020-01-0111111210.1038/s41467-019-13858-zAutomated mass spectrometry imaging of over 2000 proteins from tissue sections at 100-μm spatial resolutionPaul D. Piehowski0Ying Zhu1Lisa M. Bramer2Kelly G. Stratton3Rui Zhao4Daniel J. Orton5Ronald J. Moore6Jia Yuan7Hugh D. Mitchell8Yuqian Gao9Bobbie-Jo M. Webb-Robertson10Sudhansu K. Dey11Ryan T. Kelly12Kristin E. Burnum-Johnson13Biological Sciences Division, Pacific Northwest National LaboratoryThe Environmental Molecular Sciences Laboratory, Pacific Northwest National LaboratoryNational Security Directorate, Pacific Northwest National LaboratoryNational Security Directorate, Pacific Northwest National LaboratoryBiological Sciences Division, Pacific Northwest National LaboratoryBiological Sciences Division, Pacific Northwest National LaboratoryBiological Sciences Division, Pacific Northwest National LaboratoryCincinnati Children’s Hospital Medical CenterBiological Sciences Division, Pacific Northwest National LaboratoryBiological Sciences Division, Pacific Northwest National LaboratoryNational Security Directorate, Pacific Northwest National LaboratoryCincinnati Children’s Hospital Medical CenterThe Environmental Molecular Sciences Laboratory, Pacific Northwest National LaboratoryBiological Sciences Division, Pacific Northwest National LaboratoryImaging mass spectrometry is a powerful emerging tool for mapping the spatial distribution of biomolecules across tissue surfaces. Here the authors showcase an automated technology for deep proteome imaging that utilizes ultrasensitive microfluidics and a mass spectrometry workflow to analyze tissue voxels, generating quantitative cell-type-specific images.https://doi.org/10.1038/s41467-019-13858-z |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Paul D. Piehowski Ying Zhu Lisa M. Bramer Kelly G. Stratton Rui Zhao Daniel J. Orton Ronald J. Moore Jia Yuan Hugh D. Mitchell Yuqian Gao Bobbie-Jo M. Webb-Robertson Sudhansu K. Dey Ryan T. Kelly Kristin E. Burnum-Johnson |
| spellingShingle |
Paul D. Piehowski Ying Zhu Lisa M. Bramer Kelly G. Stratton Rui Zhao Daniel J. Orton Ronald J. Moore Jia Yuan Hugh D. Mitchell Yuqian Gao Bobbie-Jo M. Webb-Robertson Sudhansu K. Dey Ryan T. Kelly Kristin E. Burnum-Johnson Automated mass spectrometry imaging of over 2000 proteins from tissue sections at 100-μm spatial resolution Nature Communications |
| author_facet |
Paul D. Piehowski Ying Zhu Lisa M. Bramer Kelly G. Stratton Rui Zhao Daniel J. Orton Ronald J. Moore Jia Yuan Hugh D. Mitchell Yuqian Gao Bobbie-Jo M. Webb-Robertson Sudhansu K. Dey Ryan T. Kelly Kristin E. Burnum-Johnson |
| author_sort |
Paul D. Piehowski |
| title |
Automated mass spectrometry imaging of over 2000 proteins from tissue sections at 100-μm spatial resolution |
| title_short |
Automated mass spectrometry imaging of over 2000 proteins from tissue sections at 100-μm spatial resolution |
| title_full |
Automated mass spectrometry imaging of over 2000 proteins from tissue sections at 100-μm spatial resolution |
| title_fullStr |
Automated mass spectrometry imaging of over 2000 proteins from tissue sections at 100-μm spatial resolution |
| title_full_unstemmed |
Automated mass spectrometry imaging of over 2000 proteins from tissue sections at 100-μm spatial resolution |
| title_sort |
automated mass spectrometry imaging of over 2000 proteins from tissue sections at 100-μm spatial resolution |
| publisher |
Nature Publishing Group |
| series |
Nature Communications |
| issn |
2041-1723 |
| publishDate |
2020-01-01 |
| description |
Imaging mass spectrometry is a powerful emerging tool for mapping the spatial distribution of biomolecules across tissue surfaces. Here the authors showcase an automated technology for deep proteome imaging that utilizes ultrasensitive microfluidics and a mass spectrometry workflow to analyze tissue voxels, generating quantitative cell-type-specific images. |
| url |
https://doi.org/10.1038/s41467-019-13858-z |
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